tag:blogger.com,1999:blog-496263467300370264.comments2022-03-26T17:52:14.434+00:00The Y.O.R.F.Cathttp://www.blogger.com/profile/08645968914831610862noreply@blogger.comBlogger169125tag:blogger.com,1999:blog-496263467300370264.post-74347530698552537932019-09-23T15:39:23.052+01:002019-09-23T15:39:23.052+01:00I think this is one of the most significant inform...I think this is one of the most significant information for me. And i’m glad reading your article. But should remark on some general things, The web site style is perfect, the articles is really great : D. Good job, cheers<br /><a href="https://www.glentzes.gr/clubs-athens" rel="nofollow">clubs αθηνα</a>Peter shorthttps://www.blogger.com/profile/00284743026244847037noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-2282131890198441812012-08-28T21:53:16.427+01:002012-08-28T21:53:16.427+01:00I appear to have not answered you Cat, sorry. We d...I appear to have not answered you Cat, sorry. We do not yet have a double tpl mutant in moss. It would be very interesting to see the phenotype. Especially as mosses do not have roots!James Lloydhttps://www.blogger.com/profile/08219295648051788360noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-11047094668825624312012-07-30T10:03:09.499+01:002012-07-30T10:03:09.499+01:00Thanks Joe,
So the ARF family has been looked at ...Thanks Joe,<br /><br />So the ARF family has been looked at in plants. Basically 3 clades appear but within each of these there are subclades of just moss or Arabidopsis genes suggesting last time moss and Arabidopsis there were 3 ARFs. <br /><br />In the large Arabidopsis study my lab did they found ARFs 2, 9, 18 from one clade and 17 from another (which also contains ARFs 10 and 16) interacted with TOPLESS and related proteins. We checked to see if homologues of ARF2/9/18 in moss interacted and they didn't (in Y2H at least) but both of the homologous genes of ARF10/16/17 in moss interacted. I hope that answered your question.James Lloydhttps://www.blogger.com/profile/08219295648051788360noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-62278991878689592972012-07-15T16:48:35.389+01:002012-07-15T16:48:35.389+01:00Very interesting post James, good to see the blog ...Very interesting post James, good to see the blog is back in action. Is it possible to tell if the interacting ARFs from Arabidopsis and moss are orthologous? If not, do they show any common features which are not found in other ARFs?Joe Boylehttps://www.blogger.com/profile/00727421737270132119noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-65787935179806459122012-07-14T17:46:07.294+01:002012-07-14T17:46:07.294+01:00Actually, I do have a question. Maybe I missed it ...Actually, I do have a question. Maybe I missed it in the post, but do you also get a double rooted phenotype in moss when you mutate the equivalent of TOPLESS? Is this event possible in moss (I remember you telling me that the genetics of moss are not as advance as in other organisms)Cathttps://www.blogger.com/profile/08645968914831610862noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-28850868397108637122012-07-09T15:31:03.506+01:002012-07-09T15:31:03.506+01:00My master's student suggested I do it. I look ...My master's student suggested I do it. I look forward to your next post (and also your visit to Leeds). Any questions/comments?James Lloydhttps://www.blogger.com/profile/08219295648051788360noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-15916957739104240712012-07-09T11:38:44.459+01:002012-07-09T11:38:44.459+01:00Back to using the blog I see! Great stuff James, a...Back to using the blog I see! Great stuff James, about time we brought it back from the dead!Cathttps://www.blogger.com/profile/08645968914831610862noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-14696103195963390182011-10-20T16:06:33.505+01:002011-10-20T16:06:33.505+01:00epigenetics may have a neurological structure in t...epigenetics may have a neurological structure in the brain itselfkenthttps://www.blogger.com/profile/06572070413657551598noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-22038834843689372772010-03-14T12:19:51.490+00:002010-03-14T12:19:51.490+00:00Thanks for the comment.
I do not follow the fiel...Thanks for the comment. <br /><br />I do not follow the field my self. from what i know it is established small RNAs can cause DNA methylation and silencing (definitely in plants) and people are developing plants where they target both the promoter with a small RNA (so not able to target an RNA only DNA) and another small RNA to the transcript so you can get down-regulation in two ways. <br /><br />i think there is still a lot of work going on to figure the basics out. components of the pathway and mechanisms.James Lloydhttps://www.blogger.com/profile/08219295648051788360noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-81052544886212524032010-03-13T16:30:45.612+00:002010-03-13T16:30:45.612+00:00James, would you say that we are getting closer to...James, would you say that we are getting closer to having some sort of general model of epigenetics or more specifically DNA methylation? I don't really follow the literature on it or anything but I am just surprised to find that we are still having to postulate as to how it even takes place at a basic level.Menelaos Symeonideshttps://www.blogger.com/profile/05361581879935604330noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-76297132732142051662010-02-18T17:56:05.263+00:002010-02-18T17:56:05.263+00:00And now its someone else's turn!And now its someone else's turn!James Lloydhttps://www.blogger.com/profile/08219295648051788360noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-88487591446521780642009-11-25T14:28:03.435+00:002009-11-25T14:28:03.435+00:00have myself been following Ig-Nobel prizes for qui...have myself been following Ig-Nobel prizes for quite some time; actually planning to give a seminar-of-sorts too on the issue - it being relegated to a kind of class-B-research. If science is the expression of a curious mind, Ig-Nobel winners have done exactly so. And that they provide the rare dollop of humour is an added advantage :-).curiositashttps://www.blogger.com/profile/07485137374927785150noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-23457960268095332142009-11-09T01:02:39.204+00:002009-11-09T01:02:39.204+00:00http://www.guardian.co.uk/commentisfree/2009/nov/0...<a href="http://www.guardian.co.uk/commentisfree/2009/nov/07/bad-science-nott-drugs" rel="nofollow">http://www.guardian.co.uk/commentisfree/2009/nov/07/bad-science-nott-drugs</a>Menelaos Symeonideshttps://www.blogger.com/profile/05361581879935604330noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-54014569458824419382009-11-08T13:36:53.585+00:002009-11-08T13:36:53.585+00:00Thanks for the link. it is difficult for policy to...Thanks for the link. it is difficult for policy to reflect the science and public feeling but for the government to go so far off the rails with this one is what astounds me. the question is if weed is as harmful or less harmful than smoking or drinking then should we outlaw them all or make them all legal. for historical reasons i dont think we can outlaw smoking and alcohol but to legalise cannabis to me is allowing mass production of something that does cause harm (cancer if nothing else) so i am not a fan of that. <br /><br />i have seen people start smoking pot, not have any aims and waste there time doing nothing and i would like to increase that. however, i am sure that happens with alcohol hell of a lot so again historical reasons are the only reason and it is not a good one. the public would be unhappy generally if we changed it all in either direction. but clearly this was a big mistake on the policy makers for both the original change and how they dealt with this.James Lloydhttps://www.blogger.com/profile/08219295648051788360noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-90758457999184361672009-11-08T01:20:56.101+00:002009-11-08T01:20:56.101+00:00I think the government needs to justify its classi...I think the government needs to justify its classification on drugs if it is going to get any support on this issue, basically they need to either explain why cannabis is illegal if they accept that it is less dangerous than alcohol or cigarettes or why they think cannabis is more dangerous. If they don’t give a (good) reason to people like Professor Nutt then how can they blame him for holding to his view? Alan Johnson certainly didn’t try to explain why Nutt’s opinion is wrong during his Sky interview or in his letter to the Guardian and for me that is the key missing piece here. <br /><br />Btw, while I didn't think the BBC coverage was great, I liked how the guy at the end of this video makes us scientists sound so sinister.<br /><br />http://www.youtube.com/watch?v=gwJ87LEsoec&feature=PlayList&p=36B9B0BC5633C7AF&index=4Joseph Boylehttps://www.blogger.com/profile/16489640800322311480noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-15509802966083822132009-10-12T17:45:52.470+01:002009-10-12T17:45:52.470+01:00Hahaha I just read the knuckle cracking paper and ...Hahaha I just read the knuckle cracking paper and the reply to that paper, it's hilarious!Menelaos Symeonideshttps://www.blogger.com/profile/05361581879935604330noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-69637880452820705402009-10-08T01:17:25.242+01:002009-10-08T01:17:25.242+01:00Where can you find information on auxin dosages fo...Where can you find information on auxin dosages for particular plants?Anonymoushttps://www.blogger.com/profile/13241726748356466802noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-43381399356814530292009-09-22T19:14:00.133+01:002009-09-22T19:14:00.133+01:00Thank you for your blogging! I came across it whe...Thank you for your blogging! I came across it when I was looking up the meaning of "ORF" and have been browsing through it since. I appreciate your interesting topics and the way you explain them. It is very easy to follow.Melissa Hallhttp://www.forgetmenotnebraska.orgnoreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-72707839303217196772009-09-15T22:58:13.709+01:002009-09-15T22:58:13.709+01:00This week on Science's podcast they mentioned ...This week on Science's podcast they mentioned a study about some sort of model that predicted how many people will be infected, how many will die, stuff like that. I suspect that the study has been published in this week's Science. I'll try to access it and have a read through, if I canCathttps://www.blogger.com/profile/08645968914831610862noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-5354653145532808022009-09-15T21:35:24.140+01:002009-09-15T21:35:24.140+01:00Ah cheers. to add to the hysteria in Cat's hom...Ah cheers. to add to the hysteria in Cat's homeland people who worked in the airport were mostly wearing gloves and masks. I was amazed. No body seems worried in the UK right now. its on the news once a blue moon now. <br /><br />the problem with the animal studies is it is difficult to gain to much as host response from genotype is very important. a good extra control for comparison in these would be to use resurrected Spanish flu and see how fast that killed them. <br /><br />i imagine Swine flu will kill a lot but mostly because of the number of people it is infected. it is somehow spreading quickly and people have low immunity to it so it will infect a hight percentage of the pop and if it kills the same proportion of infected people as seasonal flu (or higher as these suggest) it will kill A LOT!James Lloydhttps://www.blogger.com/profile/08219295648051788360noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-25409743647826581972009-09-15T05:19:55.577+01:002009-09-15T05:19:55.577+01:00This comment has been removed by a blog administrator.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-72178206682908504322009-09-10T20:06:36.597+01:002009-09-10T20:06:36.597+01:00Thank you for the link. As I said in the post, my ...Thank you for the link. As I said in the post, my knowledge of virology is restricted to an introductory module at university and all the information in the text derives solely from my curiosity in reading about the topic. It is good to learn about reliable sources of information about this flu and viruses in general, I'll make sure I check it out.Cathttps://www.blogger.com/profile/08645968914831610862noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-33592403731690710772009-09-10T19:14:08.122+01:002009-09-10T19:14:08.122+01:00I've tried very hard to make influenza (and ot...I've tried very hard to make influenza (and other viruses) comprehensible to everyone at virology.ws. I've been working on viruses (including influenza virus) in the lab for nearly 30 years, and this is my effort to give some of that knowledge back. I encourage you to check it out.Vincent Racaniellohttp://www.virology.wsnoreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-68160588128951989182009-09-06T19:59:37.187+01:002009-09-06T19:59:37.187+01:00To be honest, I'm not really sure (but can'...To be honest, I'm not really sure (but can't be bothered to read the papers again either). The papers seem to be quite politically correct, mentioning what the WHO has been saying and stuff like that. The bottom line of all of them is 'seems to be worse than seasonal flu, must monitor to make sure it doesn't get worse'. I don't think any of them was amazingly clear on whether we really should be worried or not.<br /><br />Re n numbers, I put that question because my experiments last year always had at least n=6 mice, and is impossible to do stats on n=3 per group (per virus isolate). I understand n=3 for the macaques though. I think that this is why the paper had no stats. As I say I am no expert, but surely any more conclusive study would require higher n numbers?Cathttps://www.blogger.com/profile/08645968914831610862noreply@blogger.comtag:blogger.com,1999:blog-496263467300370264.post-20663533189586938812009-09-06T19:50:41.804+01:002009-09-06T19:50:41.804+01:00Firstly I should say this is a very well-written p...Firstly I should say this is a very well-written post. Secondly, I would have like to hear more about what the authors of those papers concluded from their results in terms of, to put it very simply, how worried we should be. Surely if the n= were so low they would have highlighted that? I know I could just read the papers for myself but this is less work for me :DMenelaos Symeonideshttps://www.blogger.com/profile/05361581879935604330noreply@blogger.com